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| PC biotin-PEG3-NHS ester Basic information |
| PC biotin-PEG3-NHS ester Chemical Properties |
| Melting point | 170 °C | | storage temp. | -20°C |
| PC biotin-PEG3-NHS ester Usage And Synthesis |
| Description | PC Biotin-PEG3-NHS carbonate ester is a unique amine reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin beads. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). | | Uses | PC biotin-NHS ester is a unique amine-reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2). |
| PC biotin-PEG3-NHS ester Preparation Products And Raw materials |
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