| HEPES Basic information |
| Product Name: | HEPES |
| Synonyms: | HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE;HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID 99+%;N-(2-HYDROXYETHYL)-PIPERAZINE-N'-2-ETHANESULFONIC ACID(4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID(HEPES-ACID));Hepes,FreeAcid(N-[2-Hydroxyethyl]Piperaz;1-HeptaneSulfonicAcidSod.SaltGr;N-(2-Hydroxyethyl)Piperazine-N'-(3-EthanesulfonicAcid);N-(2-Hydroxyethyl)Piperazine-N'-(2-EthanesulfonicAcid)(Hepes);Hepes,LowSodium,FreeAcid |
| CAS: | 7365-45-9 |
| MF: | C8H18N2O4S |
| MW: | 238.3 |
| EINECS: | 230-907-9 |
| Product Categories: | Disinfectants;Biochemistry;Good's Buffers;Buffer;ReagentsBiological Buffers;GMP;AlphabeticalMolecular Biology;Biological Buffers;BioUltra Buffers;BioUltraMolecular Biology;DNA&RNA Purification;Molecular Biology Reagents;Zwitterionic |
| Mol File: | 7365-45-9.mol |
| HEPES Chemical Properties |
| Melting point | 234-238 °C |
| Boiling point | 408℃[at 101 325 Pa] |
| density | 1.07 g/mL at 20 °C |
| vapor pressure | 0Pa at 25℃ |
| refractive index | n |
| storage temp. | 2-8°C |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH Range | 7.0 - 7.6 |
| PH | 5.0-6.5 (1Maqueous solution) |
| pka | 7.5(at 25℃) |
| Water Solubility | Soluble |
| λmax | λ: 260 nm Amax: 0.06λ: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| LogP | -3.85 at 20℃ |
| CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
| Safety Information |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 24/25-22-36-26 |
| WGK Germany | 1 |
| RTECS | TL6809000 |
| TSCA | Yes |
| HS Code | 29335995 |
| MSDS Information |
| Provider | Language |
|---|---|
| 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid | English |
| ACROS | English |
| SigmaAldrich | English |
| ALFA | English |
| HEPES Usage And Synthesis |
| Description | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| Chemical Properties | White crystalline powder |
| Uses | Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability. |
| Uses | Biological buffer. |
| Uses | HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.HEPES has been used as a component of:Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices.Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity. |
| Definition | ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2. |
| General Description | HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions. |
| Biological Activity | HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media. |
| Biological Activity | Multi purpose buffer used in biological research. |
| Purification Methods | Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.] |
| HEPES Preparation Products And Raw materials |
Please leave a message for us or use the following ways to contact us, we will reply to you as soon as possible, and provide you with the most sincere service, thank you.
日本语
한국어
Français
Deutsch
España
Türkiye